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The mean P app value for the marketed non-CNS compounds from the apical to basal compartment was about 2.1 0.6 10 6 cm.s 1 (range: 1.3 10 6 cm.s 1 to 2.9 10 6 cm.s 1 ) which is consistent with a limited passage across the cell monolayer

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These findings suggest that NNMT inhibition may influence tumor cell line phenotypes in vitro through both metabolic and epigenetic mechanisms, making 5-amino-1MQ a valuable tool for investigating the metabolic characteristics of different tumor cell lines