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"Smoking does terrible things not only to the body but also to the brain, and in some parts, disproportionately more than others." The regions most affected by smoking are the frontal cortex (involved in executive function, memory input and mood), cerebellum (coordination), corpus callosum (information bridge between the two hemispheres), and amygdala (emotional response and memory), Osborn pointed out

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ImageJ was employed to quantify the HIF-1 positive area 63