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Laboratory evidence indicates it is highly effective at reducing anxiety without the addictive properties or cognitive fog associated with traditional pharmaceutical anxiolytics

Reverse Transcription-Quantitative Polymerase Chain Reaction The total RNA content was isolated from cells or the tumor using TRIzol reagent (Invitrogen), treated with DNase I (Thermo Fisher Scientific, Cat# EN0521), and then reverse transcribed into cDNA using a Maximal First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Cat# K1622)